Most protocols use 0.45um, but I was wondering would 0.22um hurt? What is the size of the lentiviral particles? It says 80-100nm on wikipedia, which means that the 0.22um filter is large enough
Collect 0.6 mL of sample from each of the six diffusion cell receptor compartments at 30, 60, 120, 240, and 360 minutes. Diluted 0.1 mL of stock sample to respective stock sample into 1.5 mL vial containing 1.4 mL of diffusion medium and mixed well. Analyzed sample using HPLC analysis to determine PRM released at each time interval.
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Aijiren syringe filter membrane is made of polyethersulfone (PES), hydrophilic (Hydrophilic), pore size is 0.22¿ m, shell diameter is 13mm, effective membrane diameter is 11.7mm, and effective membrane area is 1.09cmº .
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Reverse: 0 psi at 25 °C 0.7 bar (10 psi) at 25 °C 0.7 bar (10 psi) at 25 °C Bubble Point at 23 °C 0.1 µm: — — ≥ 4830 mbar (70.0 psig) air with water 0.22 µm: — — ≥ 3450 mbar (50.0 psig) air with water NVR Gravimetric Extractables — — After autoclaving and a 24 hour soak in ASTM® Type 1 reagent grade water at controlled room
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